英语翻译Followingstretch,muscleswereculturedwith3H-thymidine(2\2Ci/ml)tolabelDNAsynthesisduringincubation(37◦C,5%CO2).Labelingtimewassetatoneactivationcycle(22hfor8momuscles;44hfor18momuscles)(Allenetal.,1995;

Question

英语翻译
Following stretch,muscles were cultured with 3H-thymidine
(2\2Ci/ml) to labelDNAsynthesis during incubation (37 ◦C,5% CO2).
Labeling time was set at one activation cycle (22 h for 8mo muscles;
44 h for 18mo muscles) (Allen et al.,1995; Anderson and
Pilipowicz,2002; Wozniak et al.,2003) to enable direct comparison
of activation across muscles from different aged mice.After
incubation and tendon removal,muscles were washed in cold
phosphate-buffered saline,weighed and minced over ice.DNA
extracts were prepared from homogenates as reported (Archer
et al.,2006).Aliquots were processed for scintillation counting
and dissociations per minute (dpm) were expressed relative to
DNA concentration for each extract,according to Hoechst dye
assay using a Victor microplate reader (PerkinElmer,Waltham
MA) and a standard dilution curve (5–119 ng/\2l) of calf thymus
DNA (Sigma–Aldrich,St.Louis,MO) to calibrate the assay.Satellite
cell activation is represented by the ratio of dpm/\2g DNA as
reported for in vivo experiments (Anderson et al.,2005; Archer et
al.,2006).This measure is based on observations of histological
sections of muscles following stretch.In a pilot study on wholemuscle
cultures (Wozniak et al.,2005),very few nuclei outside
the satellite-cell position on fibers stained positive for new DNA
synthesis after exposure to bromodeoxyuridine in culture (Fig.1).
The current report presents a change in activation by stretch as
percent-change in dpm/\2g DNA above the level of activation in
US EDL muscles from age-matched or the same mice in the same
experiment.

Answer 1

唉 可真不容易
下面伸展,肌肉培养用3H -胸苷
（2?次/毫升）,以labelDNAsynthesis孵化期间（37◦℃,5％的二氧化碳）.
贴标的时间定在一个激活周期（22 8mo肌肉H级;
44每小时18mo肌肉）（Allen等人.,1995;安德森和
Pilipowicz,2002;沃兹涅克等.,2003年）,可直接比较
全国肌肉激活不同年龄小鼠.后
孵化和肌腱搬迁,肌肉在冷水洗
磷酸盐缓冲液,体重超过冰碎.脱氧核糖核酸
提取液制备匀浆所报告（弓箭手
等.,2006）.等分进行处理,以计算闪烁
和每分钟器（DPM）机制分离相对表示了
DNA浓度为每提取物,根据赫希斯特染料
试验使用的是维克多酶标仪（珀金埃尔默,沃尔瑟姆
MA）和一个标准的稀释曲线（5-119纳克/?升的小牛胸腺）
脱氧核糖核酸（∑-秩序,圣路易斯,密苏里州）校准检测.卫星
细胞活化的代表是在衰变率/?克DNA作为
报告的体内实验（Anderson等.,2005年,弓箭手等
报.,2006）.这一措施是根据组织学观察
以下部分的肌肉伸展.在一项试验性研究wholemuscle
文化（沃兹涅克等.,2005年）,极少数细胞核外
卫星细胞在纤维染色的积极立场,为新的DNA
合成后接触溴文化（图1）.
本报告介绍了延伸,在激活变化
姊在DPM变化/?克以上的基因活化水平
美国的EDL肌肉年龄相仿或相同的老鼠在同一
实验.