英语翻译四种酵母经过活化后分别稀释、凃板接种于YEPD培养基,温度时间培养48h后,分别挑取单菌落于50mL液体YEPD培养基中,置于恒温摇床中在30℃,120r/min条件下培养20h,使菌体活力达到最佳.

Question

英语翻译
四种酵母经过活化后分别稀释、凃板接种于YEPD培养基,温度时间培养48h后,分别挑取单菌落于50mL液体YEPD培养基中,置于恒温摇床中在30℃,120r/min条件下培养20h,使菌体活力达到最佳.

Answer 1

After activation of four yeast were diluted,plated inoculated in YEPD medium,culture temperature and time after 48h,single colonies were picked in 50mL of liquid YEPD medium,and placed in a constant temperature shaker at 30 ℃,120r/min conditions cultured 20h,so that optimum cell viability.