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在线翻译,急,在线等,机器翻的就别进了(f)Protease.—Keeprefrigerated.Caseinassay.—300–400Units/mL.(1proteaseunitisdefinedastheamountofenzymerequiredtohydrolyze(andsolubilizeinTCA)1µmoletyrosineequival
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在线翻译,急,在线等,机器翻的就别进了
(f) Protease.—Keep refrigerated. Casein assay.—300–400 Units/mL. (1 protease unit is defined as the amount of enzyme required to hydrolyze (and solubilize in TCA) 1 µmole tyrosine equivalents/min from soluble casein at pH 8.0 and 40°C); 7–15 units/mg (1 unit will hydrolyze casein to produce color equivalent to 1.0 µmole tyrosine/min at pH 7.5 and 37°C). Color by Folin-Ciocalteau reagent. Azo-casein assay.—300–400 Units/mL [1 unit endo-peptidase activity is defined as the amount of enzyme required to hydrolyze (and solubilize in TCA) 1 µmole tyrosine equivalents/min from soluble casein at pH 8.0 and 40°C].
(g) Amyloglucosidase.—Keep refrigerated. Starch/glucose oxidase–peroxidase method.—2000–3300 Units/mL (1 unit enzyme activity is defined as the amount of enzyme required to release 1 µmole glucose/min at pH 4.5 and 40°C). PNPBM (p-nitrophenyl beta-maltosidase) method.—130–200 Units/mL (1 unit enzyme activity [PNP unit] is the amount of enzyme, which in the presence of excess levels of beta-glucosidase, will release 1 µmole p-nitrophenyl from p-nitrophenyl beta-maltosidase/min at 40°C).
The only enzyme which has been found to be significantly contaminated with interfering activities is amyloglucosidase. Thermostable alpha-amylase and protease from commercial sources have been found to be generally free of interfering enzymes. Low levels of beta-glucanase have been detected in protease preparations, but at levels well below that which would interfere with total dietary fiber analysis. The major contaminant in amyloglucosidase preparation was shown to be an endo-cellulase and resulted in endo-depolymerization of mixed-linkage beta-glucan from barley and oats, with resultant underestimation of this dietary fiber component. The contamination of amylogucosidase with endo-cellulase (beta-glucanase) can be easily detected.
Alternatively, there are kits containing all 3 enzymes (pretested) available from a number of companies.
(h) Sodium hydroxide solution.—0.275M. Dissolve 11.00 g NaOH ACS in ca 700 mL H2O in 1 L volumetric flask. Dilute to volume with H2O.
(i) Hydrochloric acid solution.—0.325M. Dilute stock solution of known titer, e.g., 325 mL 1M HCl, to 1 L with H2O.
(j) Celite.—Acid-washed.
(f) Protease.—Keep refrigerated. Casein assay.—300–400 Units/mL. (1 protease unit is defined as the amount of enzyme required to hydrolyze (and solubilize in TCA) 1 µmole tyrosine equivalents/min from soluble casein at pH 8.0 and 40°C); 7–15 units/mg (1 unit will hydrolyze casein to produce color equivalent to 1.0 µmole tyrosine/min at pH 7.5 and 37°C). Color by Folin-Ciocalteau reagent. Azo-casein assay.—300–400 Units/mL [1 unit endo-peptidase activity is defined as the amount of enzyme required to hydrolyze (and solubilize in TCA) 1 µmole tyrosine equivalents/min from soluble casein at pH 8.0 and 40°C].
(g) Amyloglucosidase.—Keep refrigerated. Starch/glucose oxidase–peroxidase method.—2000–3300 Units/mL (1 unit enzyme activity is defined as the amount of enzyme required to release 1 µmole glucose/min at pH 4.5 and 40°C). PNPBM (p-nitrophenyl beta-maltosidase) method.—130–200 Units/mL (1 unit enzyme activity [PNP unit] is the amount of enzyme, which in the presence of excess levels of beta-glucosidase, will release 1 µmole p-nitrophenyl from p-nitrophenyl beta-maltosidase/min at 40°C).
The only enzyme which has been found to be significantly contaminated with interfering activities is amyloglucosidase. Thermostable alpha-amylase and protease from commercial sources have been found to be generally free of interfering enzymes. Low levels of beta-glucanase have been detected in protease preparations, but at levels well below that which would interfere with total dietary fiber analysis. The major contaminant in amyloglucosidase preparation was shown to be an endo-cellulase and resulted in endo-depolymerization of mixed-linkage beta-glucan from barley and oats, with resultant underestimation of this dietary fiber component. The contamination of amylogucosidase with endo-cellulase (beta-glucanase) can be easily detected.
Alternatively, there are kits containing all 3 enzymes (pretested) available from a number of companies.
(h) Sodium hydroxide solution.—0.275M. Dissolve 11.00 g NaOH ACS in ca 700 mL H2O in 1 L volumetric flask. Dilute to volume with H2O.
(i) Hydrochloric acid solution.—0.325M. Dilute stock solution of known titer, e.g., 325 mL 1M HCl, to 1 L with H2O.
(j) Celite.—Acid-washed.
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(f)蛋白酶.-被冷藏的保留. 酪蛋白分析用试样.- 300-400 Units/mL. (1个蛋白酶单位被定义,当酵素要求的数量水解(和溶解在三辛胺) 1 µmole酚基乙氨酸等值或分钟从可溶解酪蛋白在酸碱度8.0和40°C); 7-15 units/mg (1个单位将水解酪蛋白导致颜色相当于1.0 µmole酚基乙氨酸或分钟在酸碱度7.5和37°C). 颜色由福-本-迈三氏法Ciocalteau试剂. Azo酪蛋白分析用试样.- 300-400 Units/mL [1单位肽链内切酶活动被定义,当酵素要求的数量水解(和溶解在三辛胺) 1 µmole酚基乙氨酸等值或分钟从可溶解酪蛋白在酸碱度8.0和40°C].
(g)淀粉葡萄糖苷酶.-被冷藏的保留. 淀粉或葡萄糖氧化酶过氧化物酶方法.- 2000-3300 Units/mL (1单位酶活性被定义,酵素要求的数量发布1 µmole葡萄糖或分钟在酸碱度4.5和40°C). PNPBM (磷硝基苯酚betamaltosidase)方法.- 130-200 Units/mL (1单位酶活性[PNP单位]是数量酵素,在beta葡糖苷酶面前的剩余水平,从磷硝基苯酚将发布1 µmole磷硝基苯酚betamaltosidase或分钟在40°C).
被发现显著沾染与干涉的活动的唯一的酵素是淀粉葡萄糖苷酶. 耐高温的α淀粉酶和蛋白酶从商业来源被发现通常免于干涉的酵素. 低水平的betaglucanase充分地低于被查出了在蛋白酶准备,但是在水平将干涉总饮食纤维分析的那. 主要污染物在淀粉葡萄糖苷酶准备证明是内纤维素酶并且起因于混杂连接beta葡聚糖的内解聚作用大麦和燕麦,与这个饮食纤维组分的总值低估. amylogucosidase的污秽与内纤维素酶(betaglucanase)可以容易地被查出.
或者,有包含全部3酵素的成套工具(预先测试)可得到从很多家公司.
(h)氢氧化钠解答.- 0.275M. 溶化11.00 g NaOH ACS在加州700 mL H2O在1升容量瓶. 稀释对容量用H2O.
(i)盐酸解答.- 0.325M. 稀释已知的滴定量,即, 325 mL的储备溶液1M HCl,到1升用H2O.
(j)钙铁石.-用酸冲洗.
(g)淀粉葡萄糖苷酶.-被冷藏的保留. 淀粉或葡萄糖氧化酶过氧化物酶方法.- 2000-3300 Units/mL (1单位酶活性被定义,酵素要求的数量发布1 µmole葡萄糖或分钟在酸碱度4.5和40°C). PNPBM (磷硝基苯酚betamaltosidase)方法.- 130-200 Units/mL (1单位酶活性[PNP单位]是数量酵素,在beta葡糖苷酶面前的剩余水平,从磷硝基苯酚将发布1 µmole磷硝基苯酚betamaltosidase或分钟在40°C).
被发现显著沾染与干涉的活动的唯一的酵素是淀粉葡萄糖苷酶. 耐高温的α淀粉酶和蛋白酶从商业来源被发现通常免于干涉的酵素. 低水平的betaglucanase充分地低于被查出了在蛋白酶准备,但是在水平将干涉总饮食纤维分析的那. 主要污染物在淀粉葡萄糖苷酶准备证明是内纤维素酶并且起因于混杂连接beta葡聚糖的内解聚作用大麦和燕麦,与这个饮食纤维组分的总值低估. amylogucosidase的污秽与内纤维素酶(betaglucanase)可以容易地被查出.
或者,有包含全部3酵素的成套工具(预先测试)可得到从很多家公司.
(h)氢氧化钠解答.- 0.275M. 溶化11.00 g NaOH ACS在加州700 mL H2O在1升容量瓶. 稀释对容量用H2O.
(i)盐酸解答.- 0.325M. 稀释已知的滴定量,即, 325 mL的储备溶液1M HCl,到1升用H2O.
(j)钙铁石.-用酸冲洗.
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