英语翻译Thecryo-EMstructureoftheT4Ssystemcorecomplexrevealedadouble-walledstructureinthecapoftheOlayer(SupplementaryFig.10a)12.Thecrystalstructureofthecapisnotdouble-walled.ThisisbecausethelipidmoietyofTraN/Vir

Question

英语翻译
The cryo-EM structure of the T4S system core complex revealed a double-walled structure in the cap of the O layer (Supplementary Fig.10a)12.The crystal structure of the cap is not double-walled.This is because the lipid moiety of TraN/VirB7 was not built (the single-wavelength anomalous dispersion (SAD)-derived map showed electron density connecting to Cys 15 of TraN/VirB7 (the lipidation site) but the density was not sufficiently well-defined forunambiguous fitting).However,when the crystal structure of the outer-membrane complex is superimposed on the cryo-EM structure of the entire complex (Fig.4c),Cys 15 of TraN aligns perfectly at the base of the outer wall of the cap,suggesting that this part of the cap might indeed contain the lipidated part of TraN (indicated in Fig.4c in black dots).

Answer 1

cryo-EM t4系统核心复杂的结构显示一个双层光学结构的帽O层(补充图10)12.晶体结构的限制不应是被称作.这是由于脂质一部分TraN / VirB7不是一天建成的(单波长反常色散(SAD)派生的地图显示电子密度连接半胱氨酸15 TraN / VirB7(lipidation网站),但没有足够明确的forunambiguous密度拟合).然而,当外膜的晶体结构复杂的叠加在cryo-EM整个结构复杂(图4 c),半胱氨酸15 TraN完全对齐的底部的外墙的帽,建议这部分的帽子也许确实包含lipidated TraN的一部分(显示在图4 c在黑点).