英语翻译Permeatesampleswererefrigeratedovernightat4◦Cpriortoanalysis.Theywereanalysedbyvariousmethodsincludingwetchemistryandsubsequentspectrophotometry,atomicadsorptionspectrometry(AAS)andhighpressureliquidchromato

Question

英语翻译
Permeate samples were refrigerated overnight at
4 ◦C prior to analysis.They were analysed by various
methods including wet chemistry and subsequent
spectrophotometry,atomic adsorption spectrometry
(AAS) and high pressure liquid chromatography
(HPLC).
Starch was analysed using the classical iodine binding
method,which although not intended as a quantitative
test could be used quantitatively with care.About 250\1l aliquots of sample were diluted to 3ml with
deionised water and 1.0 ml of 0.001Miodine test solution
(iodine/potassium iodate) was added.The starch
concentration was then deduced from the absorbance
of the test solution at 600 nm using a Perkin-Elmer
Lambda 2 spectrophotometer.Protein concentration in solution may be quantified
in a number of ways.Williams et al.[21] identified
that the pyrogallol red-molybdate (PRM) assay
was the most suitable for beer and was thus used
here.The assay was supplied by Sigma chemicals as
the microprotein-PRTM reagent.About 1.0 ml of the
reagent was added to 20 \1l of sample prior to incubating
in a water bath at 30 ◦C for 3 min.The absorbance
at 600 nm was then immediately measured (as above).The ferric chloride method (detailed by de Clerck
[22]) was used in the determination of catechin concentration
in solution.No other substances present in
the model beer solution were found to interfere with
the method.The 3ml aliquots of sample were buffered
by adding 20\1l of 0.25M sodium carbonate solution.
About 500 \1l of 0.15M iron(II) chloride was then
added prior to measuring the absorbance at 600 nm (as
above).

Answer 1

渗透样本冷藏过夜
4◦荤前的分析.他们分析了各种
包括湿化学和随后的方法
分光光度法,原子吸收光谱法
（AAS）和高压液相色谱法
法（HPLC）.
Starch was analysed using the classical iodine binding
方法,它虽然不打算作为一个定量
测试可用于定量小心.约250?升等分样品稀释至三毫升与
去离子水和1.0毫升0.001Miodine测试解决方案
（碘/碘酸钾）加入.淀粉
浓度当时推断吸光度
测试解决方案,在600 nm使用珀金埃尔默
拉姆达2 spectrophotometer.Protein在溶液中可量化
在许多方面.威廉姆斯等人.[21]确定
该邻苯三酚红钼（罗马尼亚党）法
是最适合啤酒,因此使用
这里.该法检测提供的化学品管理法
在微量-柏亚天试剂.约1.0毫升
试剂加入到20?样品升孵化前
在30◦荤水浴3分钟.吸光度
在600 nm当时立即测量（如上）.在氯化铁法（由德蛛详细
[22]) was used in the determination of catechin concentration
在溶液中.目前没有其他物质的
模型的啤酒中找到解决办法来干预
该方法.样品的三毫升等分的缓冲
加入20?的0.25米钠L碳酸盐溶液中.
约500呢?0.15M铁（Ⅱ）当时氯化升
将之前衡量在600 nm的吸光度（如
段）.