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英语翻译称取一定量的壳聚糖偶联药物,将其置于透析袋中(截留分子量8000~12000),置于pH值7.4的磷酸缓冲液(PBS),密封防挥发,水浴恒温振荡,温度37±0.5℃,转速100r/min.在设定的时间,取5.0mL溶液测

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英语翻译
称取一定量的壳聚糖偶联药物,将其置于透析袋中(截留分子量8000~12000),置于pH值7.4的磷酸缓冲液(PBS),密封防挥发,水浴恒温振荡,温度37±0.5 ℃,转速100 r/min.在设定的时间,取5.0 mL溶液测吸光度A,同时补充等量新鲜PBS溶液.对比标准曲线得缓释液中5-FuOH的浓度C,按式2计算样品在不同时间的累积释药率,绘制释药率-时间曲线.
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take a certain amount of chitosan coupling drugs,placed it in dialysis bag (intercept molecular weight 8000 12000),in pH value 7.4 phosphate buffer solution (PBS),seal and evaporation,water bath temperature oscillation,the temperature is 37 + / - 0.5 ℃,speed of 100 r/min.In the set time,take 5.0 mL solution measuring absorbance A,at the same time added amount fresh PBS solution.Contrast standard curve to slow release solution in the 5 - FuOH concentration C,according to type 2 calculation sample in different time cumulative drug release rate and draw the drug release rate - time curve.
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