分析化学英文文献翻译一段话!HighperformanceliquidchromatographyHPLCdeterminationwasperformedwithaShimadzuModelLC-6AliquidchromatographsuppliedwithaReodyneModel7125manualinjectorwitha20-μlloop.ThedetectorwasShim

Question

分析化学英文文献翻译 一段话!
High performance liquid chromatography
HPLC determination was performed with a Shimadzu Model
LC-6A liquid chromatograph supplied with a Reodyne Model
7125 manual injector with a 20-μl loop. The detector was
Shimadzu Model SPD-6A UV spectrometer. The Thermo Separation
Products integrator Model SP 4400 was used for data acquisition
and recording. Prior to use, the eluents were filtrated through
0.45 μm. filters (Sartorius) and degassed under the vacuum. The
column was reversed-phase Hypersil ODS (150×4.6 mm; participle
size 5 μm.) with a Hypersil ODS guard column (Supelco Inc.).
The beer samples were analysed for biogenic amines according to
the modified Geiger method [18]. The elution program consisted
of a gradient system with the following solvents: (A) 0.005 M.
ammonium acetate buffer solution adjusted to pH 4.3 with glacial
acetic acid; (B) methanol. After loading the sample, analysis started
with a methanol (B) gradient in buffer A from 65% to 80% for
20 min. and then with isocratic elution of 100% methanol for
10 min. Finally, the column was equilibrated with an isocratic elution
of 65% methanol in buffer A for 10 min.
The flow rate was 1 mL/min. The eluted dansylamines were
detected by monitoring the UV absorbency at 340 nm.
Results
Twenty-seven samples of popular Polish beers were tested
by HPLC method. Eight biogenic amines: monoamines
(TRP, PHM, HIS, TYR), diamines (PUT, CAD),
polyamines (SPD, SPM) and polyamine-derived secondary
metabolites (PRP, PIR) were investigated in the beer
samples. The gradient elution of methanol in ammonium
acetate buffer allowed elution of all the amines in less
than 30 min. In the tested range of biogenic amine concentration
from 0.2 to 10.0 mg/L the correlation coefficient
between peak area and concentration of each standard
amine was higher than 0.99 for every standard
curve. The average recoveries of the amines ranged from
81.6% to 104.1% with the lowest value for spermine and
the highest for spermidine. The quantification limits
were established as three times the detection limit and
PRP propylamine, PIR pyrrolidine, TRP tryptamine, PHM 2-phenylethylamine,
PUT putrescine, CAD cadaverine, HIS histamine, TYR
tyramine, SPD spermidine, SPM spermine, – not found. Unusually
最后一句"PRP propylamine, PIR pyrrolidine, TRP tryptamine, PHM 2-phenylethylamine,
PUT putrescine, CAD cadaverine, HIS histamine, TYR
tyramine, SPD spermidine, SPM spermine, – not found. Unusually”改为“ranged from 0.19 mg/L for spermidine to 0.49 mg/L for2-phenylethylamine”.！！！！！！
恳请不要乱翻译 老师要看的！！GOOGLE翻译我也会！！！

Answer 1

高效液相色谱法
高效液相色谱法测定进行了气相色谱仪模型
液晶6A条液相色谱仪提供了Reodyne模型
7125手动注射器与20 μl循环.该探测器
岛津型号的SPD - 6A条紫外光谱仪.热分离
产品集成示范警司4400用于数据采集
和记录.使用前,被过滤的洗脱液通过
0.45微米.过滤器（德国赛多利斯）和真空脱气下.那个
柱反相色谱柱消耗臭氧层物质（ 150 × 4.6毫米;词
5微米的大小.）消耗臭氧层物质的后卫色谱柱柱（ Supelco公司） .
啤酒样品进行了分析,根据生物胺
修改后的盖格法[ 18 ] .洗脱程序包括
一个梯度系统下列溶剂：（一） 0.005先生
醋酸铵缓冲溶液调pH至4.3冰川
醋酸; （乙）甲醇.装货后的样品,分析开始
以甲醇（乙）梯度缓冲甲从65 ％至80 ％的
20分钟.然后与isocratic洗脱100 ％甲醇为
10分钟.最后,柱平衡与isocratic洗脱
65 ％甲醇缓冲区A的10分.
流速为1毫升/分钟.洗脱dansylamines的人
检测监测紫外线吸光度在340 nm左右.
结果
27个样本的热门波兰啤酒进行了测试
高效液相色谱法测定方法.8生物胺：单胺类
（激进党,PHM ,他的氨基酸） ,二胺（把,计算机辅助设计） ,
多胺（社民党,扫描探针显微镜）和多胺源中学
代谢物（覆检委员会,PIR ）报告进行了调查,啤酒
样品.在梯度洗脱中甲醇铵
醋酸盐缓冲允许洗脱所有胺在不到
超过30分钟.在测试范围内生物胺浓度
0.2至10.0毫克/升的相关系数
之间的峰面积和浓度每标准
胺高于0.99每标准
曲线.平均回收率的胺不等
81.6 ％到104.1 ％ ,最低值为精胺和
最高的精.定量限
建立了3倍,检测限和
覆检委员会丙胺,红外吡咯,激进党色,PHM 2 -苯乙胺,
对PUT腐的CAD尸,他的组胺氨基酸
酪胺,社民党精,索爱精-未找到.不寻常
问题补充：
最后一句“覆检委员会丙胺,红外吡咯,激进党色,PHM 2 -苯乙胺,
对PUT腐的CAD尸,他的组胺氨基酸
酪胺,社民党精,索爱精-未找到.不寻常“改为”从0.19 mg / L时为精,以0.49 mg / L的for2 -苯乙胺".!