英语翻译2.3.CdtreatmentandplantharvestWheatseedlingsweregivensixtreatments.Theseinclude:(1)wateralone,(2)50[1]MCdCl2(∼=3.54mgCdl−1;hereafterreferredasCd),(3)250uMCdCl2(∼=17.72mgCdl−1),(4)water+200

Question

英语翻译
2.3.Cd treatmentand plant harvest
Wheat seedlings were given six treatments.These include:(1) water alone,(2) 50[1]M CdCl2(∼=3.54 mg Cd l−1; hereafterreferredas Cd),(3)250 uM CdCl2(∼=17.72 mg Cd l−1),(4)water + 200 uM SNP,(5) 50 uM CdCl2+200uM SNP,and (6)250 uM CdCl2+ 200uM.There were five replications for each treatment.Next day (after 24 h),the treatment solution was replaced with distilled waterand subjected to recovery.Plants were sampled after 24 h (Cd treatment),1 and2 days after Cd removal (i.e.48 and 72 h after initial treatment).Apicalportion(∼1.5–2.0 cm) of the roots was removed,stored at −80◦C,and used forvarious biochemical estimations.
2.4.Lipidperoxidation concentration
Lipid peroxidationin root tissue was determined by measuring malondialdehyde (MDA),a majorthiobarbituric acid reactive species (TBARS) and product of lipid peroxidation (Heathand Packer,1968).Root sample (100 mg) was homogenized in 5 ml of TCA (0.1%,w/v) followed by centrifugation at 10,000 × g for 10 min.To 1 ml aliquot ofsupernatant was added 4 ml of 0.5% TBA in 20% TCA.The mixture was incubated at95◦C for 30 min.The reaction was stopped by cooling over ice and contentscentrifuged at 10,000× g for 10 min.The absorbance of the supernatant wasrecorded at 532 nm.The value for non-specific absorbance at 600 nm wassubtracted.The amount of MDA was calculated using the extinction coefficient of155 mM−1cm−1and expressed as n mol g−1FW(fresh weight).

Answer 1

2.3.镉处理的植物收获
小麦幼苗进行了六次治疗.这些措施包括：（1）水,（2）50 [ 1 ] M氯化镉（∼= 3.54毫克镉升−1；hereafterreferredas CD）,（3）250微米的氯化镉（∼= 17.72毫克镉升−1）,（4）水+ 200微米的SNP（5）,50微米的氯化镉+ 200um的SNP,和（6）250微米镉+ 200um.有每个处理重复五次.第二天（24小时后）,用蒸馏水处理液进行回收更换.植物取样后24 h（Cd处理）,镉的去除1和2天之后（即48和72小时后开始治疗）.apicalportion（∼1.5–2厘米）的根被删除,存储在−80◦C,和用于各种生化的估计.
2.4.脂质过氧化作用浓度
通过测量丙二醛（MDA）的根组织的脂质过氧化,一majorthiobarbituric酸反应物质（TBARS）和脂质过氧化产物（heathand封隔器,1968）.根样品（100毫克）是在TCA ML 5匀浆（0.1%,W / V）在10000×g离心10分钟到1毫升等分ofsupernatant加入4毫升0.5%的TBA在20%三氯乙酸.混合培养at95◦C 30分钟.反应停的冷却冰和contentscentrifuged在10000×G 10分钟的上清液记录在532 nm处的吸光度.非特异性在600 nm处的吸光度值wassubtracted.MDA含量计算的消光系数of155毫米−1cm−1表示为n摩尔G−1FW（鲜重）.